HRP Anti-SAH Monoclonal Antibody Clone 301-16 | MAH00302-50

HRP Anti-SAH Monoclonal Antibody Clone 301-16 | MAH00302-50 | Arthus Biosystems

Product name         

HRP-anti-SAH 2a

Catalog Number     

MAH00302-50

Description              

Horseradish peroxidase (HRP) conjugated anti-S-adenosylhomocysteine monoclonal antibody clone 301-16

Specificity                

MAH00302 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: < 3%, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione: < 1%, L-Cystathionine: < 1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.

Immunogen             

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at -20°C

Storage buffer                    

PBS 10mM pH7.4 (NaCI 150mM), Proclin 0.1%, Glycerol 50%, BSA 10mg/mi

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

301-16

Immunoglobin isotype      

IgG3

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MAH00302 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. The product may be used in other not-yet-tested applications. Refer to Cat # MA00304 for details.

Notes

cELISA: 1/500 — 1/1,000 (depending on the amount of coating)

Figure 1: The standard curve with HRP-301-16 in direct competitive ELISA. The 0.5 pg/ml of SAH coating standard (Cat   # ACT00302 Lot # ACP420210) was coated on micro-titer wells. Serial dilution of SAH standard (Cat   # AST00301) was added along with 1:1,000 diluted HRP-301-16 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.