Biotin 10C & 2N S-AdenosylHomocysteine (SAH) | ACT00305

Biotin 10C & 2N S-AdenosylHomocysteine (SAH) | ACT00305 | Arthus Biosystems

Product name       

Bio-12CN-SAH 1a/b/c

Catalog Number

ACT00305-10/50/100

Description           

Biotin is conjugated with S-adenosylhomocysteine through a 10-carbon and 2-nitrogen linker.

Properties

Verification

The ninhydrin colorimetry and thin layer chromatography methods were used to show  reactions were complete and the conjugated products have the SAH component. Thorough purification was performe in each  step to ensure removal of  any non-conjugated materials. Ultraviolet absorption  spectrum  of the  conjugated  product showed  a  peak  at 256.6nm. SAH UV absorption spectrum does not show any peak.

Applications

The use of ACT00305-10/50/100 in the following application has been tested. Optimal concentrations should be determined by the end user. The product may be used in other not-yet-tested applications.                                                            

Competitive ELISA

(Plates coated with BSA-SAH, SA, Mouse-anti-SAH antibody respectively)

Notes

No difference  between   Biotin-conjugated  and unconjugated SAH molecules in their capabilities to competitively bind anti-SAH antibody.

Figure 1 : Streptavidin was coated on micro-plate, followed by Bio-12CN-SAH. HRP-anti-SAH antibody was then added and OD450 was read.