Conjugate Buffer (25 ml) | SPB25-1

Acridan conjugates. Conjugates should be prepared as described in the Lumigen SPARCL™ kit instructions. After preparation, dilute the conjugate with 3 volumes of SPB25-1 and stored as aliquots at -80°C (storage at -20°Cfor 1 week is usually ok but depends on the antibody). HRP conjugates. HRP conjugates, in azide-free buffer, should be diluted with 3 volumes of SPB25-1 and stored as aliquots at -80°C (storage for several weeks at - 20°Cis usually ok but depends on the antibody).

SPARCL™ Conjugate Buffer

Composition: A proprietary buffer for storage of acridan and HRP conjugate stocks used in SPARCL™ assays.

STORAGE 2-8°C

GENERAL INSTRUCTIONS

1. Please take the time to completely read all instructions before starting your assay. Contact us if you need clarification.

2. All reagents used in the assay should be allowed to reach room temperature (25°C) before use.

3. It is important that standards and samples be added to the SPARCL™ plate quickly. If testing large numbers of samples, rather than pipetting standards and samples directly into the white SPARCL™ plate using a single channel pipettor, we recommend the following. First, pipette an excess volume of standards and samples into appropriate wells of the clear 96- well plate. Then use an 8- or 12-channel multipipettor to quickly and efficiently transfer 50 ml aliquots to the appropriate wells of the white SPARCL™ plate. The wells of the clear plate hold a maximum volume of 300 ml.

4. Follow the sequence of events below when running the assay.