HRP Conjugation | SP-HRP

HRP Conjugation | SP-HRP from Gentaur SPARCL is in Stock.

Comments

HRP conjugated antibodies will be returned to the customer in a proprietary stabilization buffer that we use in our SPARCL™ kits. The conjugate will be shipped on dry ice and should be stored at -80°C. We find that most HRP conjugated antibodies are stable for several years at -80°C and that they withstand several freeze-thaw cycles. For optimum stability, however, we recommend that conjugates be stored as multiple aliquots after the initial thaw. Thaw the conjugate at room temperature.

Custom HRP conjugation of antibodies for use in SPARCL™ assays.

Antibody Requirement: Antibody must be provided to LDI at a concentration of 1 mg/ml or greater. Minimum Quantity 1 mg

STORAGE

Store the HRP conjugate, acridan conjugate and CRP stock at -70oC (they may be stored at -20oC for one week). The remainder of the kit should be stored at 2-8°C. The SPARCL™ plate should be kept in a sealed bag with desiccant and antioxidant. The kit will remain stable for at least six months from the date of purchase, provided that the components are stored as described above.

GENERAL INSTRUCTIONS

1. Please take the time to completely read all instructions before starting your assay. Contact us if you need clarification.

2. All reagents used in the assay should be allowed to reach room temperature (25°C) before use.

3. It is important that standards and samples be added to the SPARCL™ plate quickly. If testing large numbers of samples, rather than pipetting standards and samples directly into the white SPARCL™ plate using a single channel pipettor, we recommend the following. First, pipette an excess volume of standards and samples into appropriate wells of the clear 96- well plate. Then use an 8- or 12-channel multipipettor to quickly and efficiently transfer 50 ml aliquots to the appropriate wells of the white SPARCL™ plate. The wells of the clear plate hold a maximum volume of 300 ml.

4. Follow the sequence of events below when running the assay.