Hydrogen Peroxide (H₂O₂) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. It is involved in many pathological processes of diseases such as asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome, and immune system diseases.
Tribioscience’s ADHP-red Hydrogen Peroxide Assay is a sensitive, simple, direct, and HTS-ready colorimetric/fluorometric assay designed to measure H₂O₂ levels in biological samples. In the presence of Horse Radish Peroxidase (HRP), the ADHP-red probe reacts with H₂O₂ to produce a resorufin that appears red in color. The resorufin can be measured with absorption 570nm for colorimetric or with a highly stable fluorometric assay with maximum emission of 585nm (Ex/Em = 535/587 nm).

Applications:

• Detection of HRP activity on liquid systems such as ELISA.
• Western Blot or other immunoassays.
• Custom packaging and bulk purchase information is available upon request.

Key Features:

• Flexible: suitable fo colorimetric and fluorometric methods.
• Accurate: Use 50μL samples. Detection ranges 0.4-200μM in a 96-well plate for colorimetric and fluorometric assay.
• Simple and high-throughput: One-step procedure – Just load-incubate-read. Kit can be used for a robust method.
• Time saving: less than 30-minutes.

Kit Contents:

Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 6-months.

Fluorometric Example Standard Curve:

Colorimetric Example Standard Curve: