The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.

Applications:

This kit is used for the determination of hexosaminidase activity in biological samples.

Key Features:

Fast and Sensitive: Linear detection range (20μL sample): 0.2-50 U/L for a 30-minute reaction at 37℃.
High-Throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Kit Contents:

Storage Conditions:

This kit is shipped on ice. Store all components at -20℃ in a non-frost-free freezer. Shelf life of 12 months after receiving.

Typical Data:

This standard curve is provided for demonstration only as below figure. A standard curve should be generated for each set of samples assayed.