Tribo™ Tryptase Activity Assay | TBS2101

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.

Applications:

• Cell culture supernatants, cell lysates, or other tryptase containing supplies.
• Testing of purified tryptase enzyme, in vitro inhibitor screening.
• Cell degranulation

Key Features:

• Sensitive and Accurate: The kit detects as low as 10μM in solution.
• Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

Kit Contents:

(for 100x assays)

Storage Conditions:

Store the kit at -20℃ protected from life
Shelf life: 6 months.

Example of pNA Standard Curve

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