Tribo™ Tryptase Activity Assay | TBS2101

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SKU:
TBS2101
Availability:
In Stock
SIZE:
100 Tests
$647.57

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Description

Tribo™ Tryptase Activity Assay | TBS2101

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.

Applications:

  • Cell culture supernatants, cell lysates, or other tryptase containing supplies.
  • Testing of purified tryptase enzyme, in vitro inhibitor screening.
  • Cell degranulation

 

Key Features:

  • Sensitive and Accurate: The kit detects as low as 10μM in solution.
  • Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

 

Kit Contents:

(for 100x assays)

Storage Conditions:

Store the kit at -20℃ protected from life
Shelf life: 6 months.

Example of pNA Standard Curve

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.

Applications:

  • Cell culture supernatants, cell lysates, or other tryptase containing supplies.
  • Testing of purified tryptase enzyme, in vitro inhibitor screening.
  • Cell degranulation

 

Key Features:

  • Sensitive and Accurate: The kit detects as low as 10μM in solution.
  • Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

 

Kit Contents:

(for 100x assays)

Component Amount
5x Assay Buffer 20 mL
Tryptase inhibitor 200μL (10mM)
Tryptase Substrate 2mL (2.5mM)
pNA Standard 100μL (10mM)
Calcium Ionophore 50μL (1mM)
Tryptase Positive Control 200μL (10 μg/mL)

 

Storage Conditions:

Store the kit at -20℃ protected from life
Shelf life: 6 months.

 

Example of pNA Standard Curve

Example Tryptase Positive Control Curve

 

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