Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues. It particularly plays its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing transition from innate to acquired immunity.

Key Features

• Novel Proprietary ELISA Approach
• Fast: Only 2 hr, not 5-6 hrs.
• Simple: Sample and Detection combined into one step.
• Easy: All procedures are performed at room temperature.
• High Sensitivity: The low detection limit is 3.0pg/mL.

The levels of human IL-6 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-6 protein.

Principle of Assay

Kit Content and Storage Condition

Typical Data

This standard curve(R2=0.9999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IL-6 is typically 3.5 pg/ml.

The Intra-CV is 5.51% and the Inter-CV is 8.97 %.

Specificity

This assay recognizes natural and recombinant human IL-6. no cross-reactivity:

Human CNTF; Human G-CSF; Human gp130; Human IL-6 R; Human IL-11; Human IL-12; Human LIF; Human LIF R; Human OSM; Mouse IL-6; Mouse IL-11; Mouse IL-12.