Human TNFꭤ ELISA kit; 1 step and 1hr | TBS3235

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TBS3235
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SIZE:
1x96-Well Plate
  • Human TNFꭤ ELISA kit; 1 step and 1hr | TBS3235
  • Human TNFꭤ ELISA kit; 1 step and 1hr | TBS3235
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Description

Human TNFꭤ ELISA kit; 1 step and 1hr | TBS3235

The TriboTM Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

 

Key Features:

  • Novel Proprietary ELISA Approach
  • Fast: Only 1 hour,  not 4-5 hrs.
  • Simple: Sample and Detection combined into one step.
  • Easy: All procedures are performed at room temperature.
  • Broad Detection Range: The detection range is from 15 to 1000 pg/mL.

The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

 

Typical Data

This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human TNF-α is typically 10 pg/ml. The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human TNF-α.

No cross-reactivity: human TNF-β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF-α; Mouse TNF RI, Mouse TNF RII.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

 

 

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