Riboclear™ provides a convenient method for clean-up of total RNA. Riboclear™ procedures employed the glassfiber membrane technology for the clean-up of total RNA, instead of conventional alcohol precipitation. RNA-containing samples mixed with buffer MS are applied to mini spin column, followed by centrifugation. RNA binds to silica membrane while most of impurities pass through. The membrane is washed by buffer RNW for removal of some molecules bound nonspecifically. At last, pure RNA is eluted by RNase-free water. Riboclear™ procedure should be performed at room temperature. The eluate should be treated with care because RNA is very sensitive to contaminants, such as RNases, often found on general lab ware and dust. To ensure RNA-stability, it is recommended to store at 4℃ for immediate analysis or to freeze at -70℃ for long-term storage.

Kit Sizes:

303-150: 50 Samples

Applications:

Clean-up of total RNA, instead of conventional alcohol precipitation.

Key Features:

Riboclear™ provides an easy and rapid method for RNA cleanup or concentration from various RNA samples in just 6 minutes. Riboclear™ eliminates alcohol precipitation by binding of RNA with column, allowing rapid and convenient preparation from various samples simultaneously. Purified RNA with Riboclear™ series are free of salts and enzymes in yields reaching 95% and are suitable for dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays and other analytical procedures. Riboclear™ plus! kit provides DNase for removal of DNA and micro column for concentration of total RNA.

Storage Conditions:

Riboclear™ should be stored at room temperature. All components are stable for 1 year.