AffiVET® PRRS Virus Antibody Rapid Test

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SKU:
AFG-VGS-60
Method:
Rapid Test
Size:
40 Tests/kit
Species:
Swine
Specimen:
Serum & Whole Blood
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Description

AffiVET® PRRS Virus Antibody Rapid Test

CAT#: AFG-VGS-60
Size: 40 Tests
Method: Rapid Test
Species: Prcine
Specimen: Serum & Whole Blood

 

Material Required:

40X PRRSV Antibody Test Cards
40X Droppers
01X User Manual

 

Storage & Expiry Date:

1) Store the test kit at 4 to 30°C in dark, sealed, dry place. DO NOT FREEZE.
2) The expiry date is 24 months from the production date, which is indicated on the box.

 

Test Principal:

  • The AffiVET® Test Strip applies a colloidal gold immuno-chromatography technique for qualitative detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) antibody in porcine serum, plasma, and whole blood.
  • During testing, the PRRSV Antibody interacts with the antigen coated with colloidal gold, forming complexes that migrate across the strip.
  • Upon reaching the T-line, specific antigens on the membrane capture the complexes, resulting in the appearance of a T line.
  • The presence of both the Test line (T line) and the control line (C line) indicates a positive test result.

Sample Collection & Preparation:

Whole Blood:

  • Collect anticoagulated whole blood.
  • Test the same day.

Serum:

  • Collect blood and keep at 37°C for 1-2h.
  • Take the supernatant and centrifuge at 1500r/min for 10min then separate the serum.
  • The serum can be stored for 2-3 days at 4°C.
  • For long term storage, freeze at -20°C.

Test Procedure:

  1. Read the manual carefully.
  2. The test card and the sample need to be at room temperature before use.
  3. Take out the test card.
  4. Use the dropper to absorb the sample.
  5. Drop 2 to 3 drops into the well marked with “S”, or use Micropipette to transfer 70 µL of the sample into well mark "S".
  6. Read the results in 5 to 10 min at room temperature.
  7. The result is invalid after 15 min.

Result Interpretation:

Negative Result:

  • Presence of C-Line
  • Absence of T-Line

This means there is no PRRSV antibody in sample or the level is lower than the detection threshold.

Positive Result:

  • Presence of C-Line
  • Presence of T-Line

This confirms the presence of PRRSV antibodies in sample.

Invalid Result:

  • Absence of C-Line
  • Presence of T-Line

or

  • Absence of C-Line
  • Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

  • The test card can only be used when brought to room temperature.
  • Don’t reuse the test card or use it after the expiry date.
  • Bring the sample and all the reagents to room temperature before use.
  • When the test card is unsealed, please use it as soon as possible.
  • Take precaution while preparing the sample. Gloves and mask are required.
  • The whole blood needs to be collected in anticoagulant tube.
  • Don’t freeze the whole blood.
  • The serum sample can be stored frozen but avoid repeated freezing and thawing.
  • This product is only for in vitro rapid diagnostic veterinary use.
  • This is a qualitative test. It is not quantitative.
  • The test results are for reference only. For confirmation, please refer to the relevant national standard methods.

 

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