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Bovine BTB Ab ELISA Kit

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SKU:
173-EB43-03
AUD1,298.00

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Description

The BIONOTE BTB Ab ELISA 2.0 is a direct Enzyme Linked Immunosorbent Assay for the qualitative detection of Mycobacterium bovis antibody in serum.
The BIONOTE BTB Ab ELISA 2.0 contains a microplate, which is pre-coated with purified BTB antigen on the well. For testing, ELISA plates coated with the antigen are incubated with an equal mixture of serum and M.Bovis antigen-HRP conjugate for 60 minutes at 37℃. During the incubation, M. bovis antibodies present in test sample bind to purified M. bovis antigen pre-coated in the well and conjugate. Following this incubation, all unbound material is removed by aspiration and washing before adding a substrate solution. The residual enzyme activity found in the well will thus be directly proportional to the conjugate concentration in specimens and evidenced by incubating the solid-phase with a substrate solution. The reaction is stopped by addition of the stopping solution and colorimetric reading should be performed by using a spectrophotometer at 450 ㎚ with reference wavelength at 620 ㎚.
The specially selected M. bovis antigens are used as capture material in the test. These enable the BIONOTE BTB Ab ELISA 2.0 to identify to M. bovis antibodies in specimens, with a high degree of accuracy.

BIONOTE BTB Ab ELISA Brochure

BIONOTE BTB Ab ELISA Insert

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Additional Information

Test Type:
ELISA
Packing Size:
480 wells/Kit
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1 Review

  • 5
    BOVINE BTB AB ELISA KIT | EB43-03 | BIONOTE

    Posted by Ford on 5th Dec 2021

    The BIONOTE BTB Ab ELISA 2.0 is a direct Enzyme Linked Immunosorbent Assay for the qualitative detection of Mycobacterium bovis antibody in serum. The BIONOTE BTB Ab ELISA 2.0 contains a microplate, which is pre-coated with purified BTB antigen on the well. For testing, ELISA plates coated with the antigen are incubated with an equal mixture of serum and M.Bovis antigen-HRP conjugate for 60 minutes at 37℃.

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