FMD NSP Ab ELISA Kit 2.0

The BIONOTE FMD NSP Ab ELISA is a Competitive Enzyme Linked Immunosorbent Assay for the qualitative detection of antibody to FMP NSP in serum or plasma of cow, pig or goat. It contains a microplate, which is pre-coated with recombinant 3ABC antigen on the well. For testing, ELISA plates coated with the 3ABC are incubated with an equal mixture of sample and monoclonal antibody-HRP for 90 minutes at 37℃. During first incubation, if antibodies against 3ABC present in the test sample, the antibodies and HRP conjugated monoclonal antibodies against 3ABC competitively bind to the antigens on the well. After incubation, all unbound materials are removed by washing step and substrate is dispensed. The enzyme activity found on the well will thus be directly inversely proportional to the anti-3ABC antibodies in serum or plasma. After incubation at room temperature, the reaction is stopped by addition of the stop solution and color development is measured optically at 450nm and 620nm.
The highly specific selected 3ABC antigens are used as capture material in this test. These enable the FMD NSP Ab ELISA to identify FMDV antibodies in serum or plasma, with a high degree of accuracy.

FMD NSP Ab ELISA Brochure

FMD NSP Ab ELISA Insert

FMD NSP Ab ELISA DataSheet