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Viral Nucleic Acid Extraction Kit I (50prep), (With Carrier RNA, for low viral load specimen using carrier RNA) | APVNK 050

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904-APVNK 050
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Description

Viral Nucleic Acid Extraction Kit I (50prep), (With Carrier RNA, for low viral load specimen using carrier RNA) | APVNK 050 | Alphagen Biotech

Component

APVNK 004
(4 preps)
APVNK 050
(50 preps)
APVNK 100
(100 preps)
APVNK 200
(200 preps)
VNE Buffer 1.8 ml x 2 35 ml 70 ml 140 ml
Wash Buffer 1 * (concentrate) 0.9 ml x 2 22 ml 44 ml 88 ml
Wash Buffer 2 * (concentrate) 1.5 ml 20 ml 40 ml 40 ml x 2
RNase-free Water 0.5 ml 6 ml 6 ml x 2 20 ml
Carrier RNA 0.05 mg 0.4 mg 0.8 mg 1.6 mg
VNE Column 4 pcs 50 pcs 100 pcs 200 pcs
Collection Tube 8 pcs 100 pcs 200 pcs 400 pcs
Elution Tube 4 pcs 50 pcs 100 pcs 200 pcs
User Manual 1 1 1 1

Brief Prodecure

Specification

  • Principle : spin column (silica membrane)
  • Sample size : 150 µl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant 
  • Fragment size : 100 bp ~30 kb
  • Recovery rate : 80 ~ 90 %
  • Binding capacity : 30 ug
  • Elution volume : 40 ~ 50 µl 
  • Operation time : 20 min

Important Notes:

  • Make sure everything is RNase-free when handling this system.
  • Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
  • Add 1 ml of VNE Buffer to the tube of lyophilized Carrier RNA, mix well by vortexing and transfer the mixture to the VNE Buffer when first open. Store the Carrier RNA added VNE Buffer at 4 °C.
  • Add required ethanol (96-100%) to Wash Buffer 1 and Wash Buffer 2 before use.
  • Preheat RNase-free water to 70˚C for elution step.

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