Sicastar® | 43-48-103
- SKU:
- MMD-43-48-103
- Availability:
- 5 Working days
- Surface:
- Ni-NTA
- Concentration:
- 50mg/ml
- Crowd:
- 10ml
Description
Sicastar® | 43-48-103
Ni-NTA
Sicastar® particles are available with the nickel(II)-nitrilotriacetic acid (Ni-NTA) complex in the size range from 300 nm to 20 µm for direct use in binding histidine-labeled proteins. Recombinant proteins containing a 6xhistidine tag can be purified using Ni-NTA chromatography. This purification is based on the interaction between nickel(II) ions on the particle surface and histidine side chains of the protein. The immobilized 6xhistidine-tagged protein can be washed and eluted by adding imidazole or EDTA or by lowering the pH.
The particles are supplied as a suspension in water without the addition of detergents.
Surface: Ni-NTA
Concentration: 50mg/ml
Crowd: 10ml
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