CheKine™ Superoxide Dismutases (SOD) Assay Kit | KTB1030

Product Overwiew:

Abbkine CheKine™ Superoxide dismutase (SOD) Activity Assay Kit is designed for the quantitative determination of SOD enzyme activity.

Background:

Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide anion into O2 and H2O2. They are an important antioxidant defense against the toxicity of superoxide radical in all cells exposed to O2. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Three forms of superoxide dismutase are present in humans. SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular.

Used for:

N/A

Kit components:

• Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• SOD Standard (200 U/mL)
• Xanthine Oxidase
• Xanthine

Features & Benefits:

• Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 3.13- 200U/mL.

Usage Notes:

• Briefly centrifuge small vials at low speed prior to opening.
• Prior to assay, bring all reagents to room temperature (25°C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting.
• If not assayed immediately, samples can be stored at -80°C.

Storage buffer:

N/A

Storage instructions:

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.