Human Fibroblast growth factor 2 (FGF2) ELISA Kit | KTE62722

Product Overwiew:

This Human Fibroblast growth factor 2 (FGF2) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF2.

Background:

FGF basic is one of at least 22 mitogenic proteins of the FGF family, which show 35 - 60% amino acid conservation.Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21 - 23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells.The 18 kDa rat sequence has 98% aa identity with mouse, and 96 - 97% identity with human, bovine and sheep FGF basic.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Fibroblast growth factor 2 (FGF2) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF2 in samples. An antibody specific for FGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Fibroblast growth factor 2 microplate
• Human Fibroblast growth factor 2 standard
• Human Fibroblast growth factor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Fibroblast growth factor 2 (FGF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human FGF2. No significant cross-reactivity or interference between Human FGF2 and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.