PurKine™ Protein A Packed Column | BMC20500

Product Overwiew:

PurKine™ Recombinant Protein A Antibody Purification Packed Column

Background:

PurKine™ Protein A Purification system is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Recombinant protein A is produced in E. coli and functions essentially the same as native Protein A from Staphylococcus aureus. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species. Protein A is generally preferred for affinity purification of rabbit, Guinea pig, dog and cat IgG. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein A Purification Resin is also available as prepacked spin column and kit formats.

Used for:

N/A

Kit components:

N/A

Features & Benefits:

• High capacity—More than 40mg human lgG per mL of resin.
• High performance—Native protein A has five IgG binding domains and many other domains with unknown functions.Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.

Usage Notes:

N/A

Storage buffer:

Liquid resin in PBS buffer containing 20% ethanol were prepacked in columns.

Storage instructions:

Stable for one year at 2-8°C from date of shipment. Do not freeze.

Shipping:

Blue ice

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.