abm | saCas9 Nuclease NLS Protein | K153
- 5 to 7 Days Shipment
ABMGood | saCas9 Nuclease NLS Protein | K153
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. This saCas9 Nuclease NLS protein is offered as lyophilized powder for better long term storage and ambient temperature shipping. The protein is lyophilized as a low-salt powder, so it can be resuspended in your desired buffer for downstream applications.
Lyophilized powder. Enzyme supplied with 10X Reaction Buffer.
saCas9, CRISPR-associated endonuclease Cas9 from Staphylococcus aureus
Store all components at -20°C.
After resuspension in 125 µl nuclease-free dH2O: 20mM HEPES, pH 7.5, 100mM KCl, 1mM DTT, 0.1mM EDTA, 1% sucrose.