abm | saCas9 Nuclease Protein | K144

ABMGood | saCas9 Nuclease Protein | K144

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).

The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

Concentration:

10 µM, 1.30 mg/ml

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

saCas9, CRISPR-associated endonuclease Cas9 from Staphylococcus aureus

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

N/A

Application:

N/A

Components:

N/A

Inactivation:

N/A

Source:

E. coli

Shipping conditions:

Ice Packs

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.