abm | Immortalized Human Astrocytes, fetal - SV40 | T0280

abm | Immortalized Human Astrocytes, fetal - SV40 | T0280

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Fetal Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

49 - 59 hours

Seeding Density:

40,000 - 50,000 cells per cm10². Recommended split ratio: 1:2

Markers:

N/A

Donor Age:

18 weeks gestation

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, 10ng/ml Human EGF (Z100135), 1% L-glutamine , and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C