Description
abm | Immortalized Human Cardiac Fibroblasts | T0446
Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen BioSafety Level: II Organism: Homo sapiens Species: Human Source Organ: Ventricles (Adult Heart) Organ Type: Heart Growth Properties: Adherent Morphology: Multipolar Passage Number: N/A Population Doubling: 48-72 hours Seeding Density: 3,500-7,000 cells/cm10² Markers: N/A Donor Age: N/A Donor Gender: N/A Donor Ethnicity: N/A Propagation: The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, nonessential amino acids (1 mM), L-glutamine (2mM), insulin (5µg/mL) (Z101065) and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Quality Control: Real Time PCR was used to quantify the SV-40 transgene expression. Shipping Condition: Dry Ice Storage Condition: liquid nitrogen or -180C
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.