abm | Immortalized Human Cardiac Fibroblasts | T0446

abm | Immortalized Human Cardiac Fibroblasts | T0446

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Ventricles (Adult Heart)

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

48-72 hours

Seeding Density:

3,500-7,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, nonessential amino acids (1 mM), L-glutamine (2mM), insulin (5µg/mL) (Z101065) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the SV-40 transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C