abm | Immortalized Human Colon Cells | T0570

abm | Immortalized Human Colon Cells | T0570

abm’s Immortalized Human Colon Cells are obtained from human normal colon section and are ideal research models for malignant and non-malignant colorectum diseases.

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Colon Section

Organ Type:

Digestive

Growth Properties:

75% Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

48-72 hours

Seeding Density:

20,000-25,000 cells/cm10²; Recommended split ratio is 1:3

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%. This cell line needs to be cultured in collagen coated flasks.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C