abm | Immortalized Human Endometriotic Cell Line (12Z) | T0764

abm | Immortalized Human Endometriotic Cell Line (12Z) | T0764

in vivo. Immortalized Human Endometriotic Cell Line (12Z) may be used to characterize and identify genes and/or markers related to endometriosis.

Immortalization Method:

Immortalized with SV40 virus

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Uterus

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

55 - 65 hours

Seeding Density:

15,000 - 20,000 cells/cm10²

Markers:

SV40T, cytokeratin, vimentin, N-cadherin, estrogen receptor α and β, progesterone receptor, and aromatase P450

Donor Age:

37 years old

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescence analysis; 2) Western blot; 3) RT-PCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C