abm | Immortalized Human Laryngeal Posterior Commissure Cells | T0499

abm | Immortalized Human Laryngeal Posterior Commissure Cells | T0499

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Larynx

Organ Type:

Digestive

Growth Properties:

Adherent

Morphology:

Epithelial (<10% fibroblasts)

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

20,000 - 30,000 cells per cm10².

Markers:

CK5, CK8

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The medium for this cell line is Prigrow X.1 medium available at abm. TM010.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C