abm | Immortalized Human Lymphoblastoid Cells – EBV (KMS-15) | T0133

abm | Immortalized Human Lymphoblastoid Cells – EBV (KMS-15) | T0133

The KMS-15 cells are positive for both CD19 and CD20 B cell antigens as well as HLA-DR. In contrast, another lyphoblastoid cell line, KMS-9 (Cat#T0132) is positive for HLA-DR and CD19B while tested negative for CD20.

Immortalization Method:

Epstein-Barr Virus (EBV)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Peripheral blood

Organ Type:

Blood

Growth Properties:

Suspension

Morphology:

Round|Clusters

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C