abm | Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT/SV40 | T0283

abm | Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT/SV40 | T0283

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen, Bmi-1 and hTERT genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Oflactory Bulbs

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

20-24 hours

Seeding Density:

5,000 cells/cm2

Markers:

S100β, GFAP, ErbB2, Neuroligin-3, Amyloid Precursor Protein, Vimentin

Donor Age:

58

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, pituitary extract to 20ug/ml, forskolin to 2uM and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunofluorescence staining for characteristic OEG markers were analyzed

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C