abm | Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT/SV40 | T0283
- SKU:
- T0283
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT/SV40 | T0283
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen, Bmi-1 and hTERT genes
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Oflactory Bulbs
Organ Type:
Brain
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
20-24 hours
Seeding Density:
5,000 cells/cm2
Markers:
S100β, GFAP, ErbB2, Neuroligin-3, Amyloid Precursor Protein, Vimentin
Donor Age:
58
Donor Gender:
Male
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow IV medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, pituitary extract to 20ug/ml, forskolin to 2uM and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Immunofluorescence staining for characteristic OEG markers were analyzed
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C