abm | Immortalized Human Skeletal Muscle Cells | T0034

abm | Immortalized Human Skeletal Muscle Cells | T0034

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Skeletal Muscle

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

30-40 hours

Seeding Density:

20,000-35,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

7

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available in abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Split Ratio: 1:2 or 1:3 recommended when subculturing.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C