abm | Immortalized Mouse Achilles Tendon Cells (iMAT) | T0035

abm | Immortalized Mouse Achilles Tendon Cells (iMAT) | T0035

Immortalization Method:

Serial passaging and co-transfection with piggyBac vector MPH86 expressing SV40 T Ag flanked with FLP and piggyBac transposase expression vector

BioSafety Level:

II

Organism:

CD1 Mouse

Species:

Mouse

Source Organ:

Achilles tendon

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

10,000 - 30,000 cells / cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C