abm | Immortalized Mouse Intestinal Myofibroblasts | T0565

abm | Immortalized Mouse Intestinal Myofibroblasts | T0565

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

C57BL/6J Mouse

Species:

Mouse

Source Organ:

Colon

Organ Type:

Digestive

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

25 - 35 hours

Seeding Density:

20,000 cells/cm10²

Markers:

α-smooth muscle actin, vimentin, type I collagen

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Western blot and immunofluorescence staining were used to determined cell markers and protein expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C