Description
abm | Immortalized Rat Portal Myofibroblasts (RGF) | T0068
Immortalization Method:
Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector
BioSafety Level:
II
Organism:
Sprague-Dawley Rat
Species:
Rat
Source Organ:
Liver
Organ Type:
Liver
Growth Properties:
Adherent
Morphology:
Spindle shaped
Passage Number:
N/A
Population Doubling:
13 - 23 hours
Seeding Density:
15,000 - 20,000 cells/cm10²
Markers:
alpha smooth muscle actin, type I collagen alpha-1, tissue inhibitor of metalloproteinases-1, PF-specific markers elastin, type XV collagen alpha-1, Ntpdase2/Cd39l1, and ecto-5’-nucleotidase/Cd73
Donor Age:
Adult
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1.) Gene expression analysis to confirm the expression of common liver myofibroblast-associated genes. 2.) Immunoblot and immunofluoresence microscopy to detect myofibroblast-specific cell markers. 3.) Transfection assay to confirm DNA transfectability of cells. 4.) Co-culture assay to demonstrate the inhibition of Mz-ChA-1 cholangiocarcinoma proliferation.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C