abm | Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11) | T0672

abm | Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11) | T0672

Immortalization Method:

Transfection with pY3 plasmid

BioSafety Level:

II

Organism:

BALB/c mouse

Species:

Mouse

Source Organ:

Mammary glands

Organ Type:

Breast

Growth Properties:

Adherent

Morphology:

Cuboidal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Recommended split ratio of 1:5

Markers:

Hygromycin B resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to final concentration of 10%, 50 µg/ml gentamicin , 5 µg/ml insulin , 10 ng/ml epidermal growth factor , and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Immunofluorescence using anti-casein antibody; 2) Southern blot for β-casein

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C