Description
abm | Immortalized Porcine Alveolar Macrophage Cells (iPAM61) | T0755
Immortalization Method: Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit BioSafety Level: II Organism: Pig (Porcine) Species: Pig (Porcine) Source Organ: Lung Organ Type: Lung Growth Properties: Adherent Morphology: Fibroblast-like Passage Number: N/A Population Doubling: N/A Seeding Density: N/A Markers: Puromycin resistance Donor Age: 10 weeks old Donor Gender: Not disclosed Donor Ethnicity: Not disclosed Propagation: The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C