abm | Immortalized Rat Microglia-SV40 | T0246

abm | Immortalized Rat Microglia-SV40 | T0246

Immortalization Method:

Serial passaging and transduction with replication incompetent recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Rattus rattus

Species:

Rat

Source Organ:

Rat brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Stellate shaped

Passage Number:

N/A

Population Doubling:

24-34 hours

Seeding Density:

30,000 cells/cm10²; Recommended split ratio is 1:2

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, 4.5g/L D- Glucose (in addition to 4.5g/L D-glucose already present in Prigrow III) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C