abm | Immortalized Rat Tracheal Epithelial Cells (SPOC-1) | T0733

abm | Immortalized Rat Tracheal Epithelial Cells (SPOC-1) | T0733

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Rat

Species:

Rat

Source Organ:

Trachea

Organ Type:

Trachea

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

~24 hours

Seeding Density:

16,000 cells/cm²

Markers:

Cornifin, keratins 13, 14, and 19, mucin, transglutaminase I

Donor Age:

10 - 14 weeks old

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow XII Medium available at abm. Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Karyotyping; 2) Tumorigenicity analysis; 3) Immunostaining; 4) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C