abm | BAK Stable Knockdown HN12 Cell Line | T6416

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SKU:
T6416
Availability:
5 to 7 Days Shipment
Size:
10⁶ cells/1.0 ml
£4,773.91

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Description

abm | BAK Stable Knockdown HN12 Cell Line | T6416

When cells undergo death triggered by chemotherapeutic agents, the BCL-2-family-dependent mitochondrial apoptotic pathway is activated. By activating BAX and/or BAK, one of the BCL-2 subgroups, BH3-only proteins cause cytochrome to release from the mitochondria. shRNAs were introduced specific for BAK and BAX in HN12 cells so their roles can be studied. The results suggest that both BAX and BAK are required for apoptosis induced by simultaneous inhibition of BCL-2.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Other

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 36 hours

Seeding Density:

N/A

Markers:

Down-regulated BAK Vector information: shBAK: TRCN0000033466 TG GTACG AAGATTCTTC AAAT

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Immunoprecipitation; 2) Western blot; 3) FACS; 4) Crystal violet assay; 5) Cell viability assays for Bliss Independence analysis

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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