abm | TSPO Stable Knockdown Huh7 Cell Line | T6402

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SKU:
T6402
Availability:
5 to 7 Days Shipment
Size:
10⁶ cells/1.0 ml
Select Currency //4,173.00

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Description

abm | TSPO Stable Knockdown Huh7 Cell Line | T6402

TSPO plays a critical role in the segregation and transport of free cholesterol (FC) from the outer to the inner mitochondrial membrane in cells. Wild type Huh7 (WT) is a well-differentiated human hepatocyte-derived carcinoma cell line, which contains TSPO in mitochondria. The stable TSPO knockdown (TSPO KD) in the Huh7 cell line was made to mimic hepatic steatosis in vitro.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 hours

Seeding Density:

80,000 - 100,000 cells/cm2

Markers:

Vector information: PBR shRNA plasmid (TSPO KD) Drug resistance: Puromycin (2μg/ml)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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