Description
abm | TSPO Stable Knockdown Huh7 Cell Line | T6402
TSPO plays a critical role in the segregation and transport of free cholesterol (FC) from the outer to the inner mitochondrial membrane in cells. Wild type Huh7 (WT) is a well-differentiated human hepatocyte-derived carcinoma cell line, which contains TSPO in mitochondria. The stable TSPO knockdown (TSPO KD) in the Huh7 cell line was made to mimic hepatic steatosis in vitro.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Liver
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
24 hours
Seeding Density:
80,000 - 100,000 cells/cm2
Markers:
Vector information: PBR shRNA plasmid (TSPO KD) Drug resistance: Puromycin (2μg/ml)
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) Western blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C