abm | αA-crystallin Stable Expressing CHO Cell Line | T6236

abm | αA-crystallin Stable Expressing CHO Cell Line | T6236

αA-crystallin is a molecular chaperone, an antiapoptotic protein, is a structural protein of the lens, and belongs to the small heat shock protein (sHSP) superfamily. To further discover the human αA-crystallin, αA-crystallin Stable Expressing CHO Cell Line was constructed for further research. This cell line may be used alongside with other stable CHO cell lines expressing the mutant αA-crystallin proteins (R12A or R49A).
R12A αA-crystallin Stable Expressing CHO Cell Line (T6234) and R49A αA-crystallin Stable Expressing CHO Cell Line (T6235) are also available from abm.

Biosafety:

II

Organism:

Hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

αA-crystallin, G418 (400 mg/ml)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow XII medium available at abm

Preservation:

1. Freeze Medium: 95% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Apoptosis induction tests

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C