abm | R12A αA-crystallin Stable Expressing CHO Cell Line | T6234

abm | R12A αA-crystallin Stable Expressing CHO Cell Line | T6234

R12A αA-crystallin Stable Expressing CHO Cell Line was designed to express the mutant R12A αA-crystallin. This cell line can be used to study the effects of the mutation on the protein's function as a molecular chaperone, an antiapoptotic protein, and as a structural protein of the lens.
αA-crystallin Stable Expressing CHO Cell Line (T6236) and R49A αA-crystallin Stable Expressing CHO Cell Line (T6235) are also available from abm.

Biosafety:

II

Organism:

Hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

R12A αA-crystallin, G418 (400 mg/ml)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow XII medium available at abm

Preservation:

1. Freeze Medium: 95% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Apoptosis induction tests

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C