abm | E-Cre-ERT2 Stable Expressing Rat1 (AD6) Cell Line | T6169

abm | E-Cre-ERT2 Stable Expressing Rat1 (AD6) Cell Line | T6169

This rat cell line expresses a chimeric molecule containing a Δ E-like fragment of Notch1 fused to a vesicular stomatitis virus epitope, the Cre recombinase, and a modified hormone binding domain of the estrogen receptor. The delivery of Cre recombinase depends on any event, including γ-secretase processing, that releases the intracellular domain of Notch1 from the membrane. Nuclear activity of the Cre-ER-T2 chimera depends on the presence of tamoxifen. Reporter construct for the recombinase activity consists of an antibiotic resistance gene (hygromycin). This cell line would be well-suited for research regarding the functions of the Notch receptor, γ-secretase enzyme activity, γ-secretase multiprotein complex, and their involvement in cellular signalling.

Biosafety:

II

Organism:

Rat (R. norvegicus)

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Neomycin resistant if not treated with tamoxifen. Hygromycin resistant if treated with tamoxifen.

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Immunofluorescence; 2) Northern blot; 3) DNA sequencing

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C