abm | IP6K2<sup>---<-sup> Stable HCT116 Cell Line | T6350

abm | IP6K2^{---<-sup> Stable HCT116 Cell Line | T6350}

To study the role that PP-IPs play in mammalian phosphate homeostasis, researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs.In IP6K2-/- cells, IP7 was significantly reduced compared with WT (wildtype) but was still present. Researchers chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only and has easily detectable levels of IP7 and IP8.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Colon

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

IP6K2 knockout

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) PCR; 2) Sequencing

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C