abm | Stable FUT8 Over-expressing HEK293S GnT I--- Cell Line | T6010

abm | Stable FUT8 Over-expressing HEK293S GnT I--- Cell Line | T6010

Human HEK293S GnT I-/- parental cell line was infected with recombinant lentivirus carrying the FUT8 gene in the pLV-CT vector to establish the Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line. FUT8 gene encodes for the fucosyltransferase 8 enzyme (α1,6-fucosyltransferase) which is involved with the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. It has been linked that FUT8 can independently fucosylate high mannose N-glycans without GnTI. Manipulated to over express FUT8, these cells can be utilized for studies relating to fucosylation and the involvement of the enzyme.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

3x10⁵ cells/ml

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

FUT8 over expressed via lentiviral infection

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is 50:50 mix of EX-Cell Sigma) and FreeStyle^TM 293 Medium Life Technologies). Add 1 µg/ml puromycin

Preservation:

N/A

Quality Control:

Puromycin resistance determined (1 µg/ml puromycin)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C