abm | Stable HEK293 Over Expressing Ras Protein Cell Line | T6012

abm | Stable HEK293 Over Expressing Ras Protein Cell Line | T6012

Stable HEK293 Over Expressing Ras Protein Cell Line is designed as a valuable tool to cultivate serotypes 40 and 41 of enteric human adenoviruses (HAdVs) for research purposes. HAdVs have been linked to drinking water contamination which is associated with waterborne diseases. Studying HAdVs can be limited due to low detection levels in samples obtained, but with the Stable HEK293 Over Expressing Ras Protein Cell Line researchers have a tool which is highly competent of supporting HAdV growth to allow more greater detection for the virus. Infect the cells with the HAdV strains and viral cDNA is amplified.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

H-RasV12-expressing retroviral vector pBABE was electroporated into HEK293 cell suspension and was selected for with G418

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm

Preservation:

N/A

Quality Control:

1) Selection with 1 mg/ml G418, 2) Ras expression analyzed via Western blot, PCR, and RT-PCR methods

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C