Methotrexate Forensic ELISA Kit | 107519

Neogen’s Methotrexate ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Methotrexate. The kit was designed for screening purposes and is intended for forensic use only.

Cross Reactivity

Materials Provided

1. EIA Buffer
2. Wash Buffer Concentrate (10X)
3. K-Blue Substrate
4. Drug-Enzyme Conjugate
5. Antibody Coated Plate
6. Red Stop Solution
7. Qualitative QC Positive Control
8. Qualitative QC Negative Control

Materials Required But Not Provided

1. Deionized water.
2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
3. Graduated cylinder to dilute and mix wash buffer.
4. Plate cover or plastic film to cover plate during incubation.
5. Clean glassware (i.e. test tubes) to dilute samples.
6. Microplate reader with 650 nm filter if Red Stop is used, or a 450 nm filter if 1N HCl is used to stop the reaction. Note: It is not necessary to stop the reaction if reading immediately. Unstopped reactions should be read with a 650 nm filter.

Optional Materials

1. 1N HCI if Red Stop Solution is not used
2. Microplate shaker