This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Features
lack of Lon and Omp proteases allows high-level protein expression and accumulation
Easy gene induction
Applications
IPTG induced gene expression in E. coli
Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
Description
Intact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical...
Description
Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.
Specifications
Competent cell type: ...
Description
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.
Specifications
Competent cell type: ...