This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Features
lack of Lon and Omp proteases allows high-level protein expression and accumulation
Easy gene induction
Applications
IPTG induced gene expression in E. coli
Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
Description
Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.
Specifications
Competent cell type: ...
Description
Intact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical...